ΕUROPEAN CENTER FOR GENETICS & DNA IDENTIFICATION

SCREENING TESTS
Εντοπισμός & Προσδιορισμός της Φύσης των Βιολογικών Υλικών
Instructions
INSTRUCTIONS FOR BUCCAL CELL COLLECTION: DNALOGY-KIT
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In the course of sampling, DNAlogy offers you free-of charge the DNAlogy-kit including the sterilized cotton swab, sampling instructions and a consent/order form for the applicant.
The aim of sampling is to collect as many cheek cells as possible and to transfer them to the sterilized cotton swab, which is going to protect DNA until its transfer to our laboratory.
Attention: Avoid any contact with the cotton tip end of the swabs.
Do not eat, drink or smoke for at least 30 minutes before sampling.
For the sampling procedure, each individual should use 2 swabs.

Step 1
Label the swabs with a unique identifying name and sampling date.

Step 2
Carefully open the sterile swab packaging holding the red plastic applicator end and remove the swab from the packaging.

Step 3
Sampling: Rub the cotton tip of the swab firmly against the inside of both cheeks several times (20-30 seconds) in each direction.

Step 4
Air dry the swabs for at least 1 hour at room temperature while keeping them protected from any source of contamination. When dry, place the swabs back in their packaging. Repeat the steps 1-4 with the second swab

Step 5
Send the swabs back to DNAlogy along with the completed questionnaire (consent and order form) by using the reply envelope.

SCREENING TESTS

Before DNA extraction and analysis of the different evidence, we first need to detect the biological material to be analyzed, a process that is not always easy and requires meticulous work, but mostly great expertise.
DNAlogy is the first laboratory in Greece that uses Screening tests before each analysis aiming at the identification of unrecognized biological traces and therefore the use of appropriate methods of sampling and analyzing DNA.

The following screening tests are applied in our lab:

To define the nature of the biological sample:
1) CRIMESCOPE :Crimescope emits UV light (400-450 nm) that allows detection of biological fluids (sperm, saliva, sweat but not blood) and fluorescence at different wavelengths.
2) TEST PHADEBAS :This test allows detection of hidden stains (saliva) by determining the activity of the « α-Amylase» enzyme.

To detect blood stains:
1) BLUE-STAR
:Blue-Star reveals blood stains that are invisible to the naked eye. Luminol, the agent of Blue-Star, produces a chemo-luminescent reaction when coming into contact with blood, emitting a blue light that is visible in the dark. This test is applied in dark areas where blood stains are not visible.
2) MEYER TEST :It is the basic test for detection of blood stains, which is based on an oxidation reaction that leads to a visible bright pink color.
3) HEXAGON-OBTI TEST : It allows detection of human hemoglobin i.e. determines if a blood sample is human or not.

To detect sperm:
1) PSA TEST, PA TEST, «CHRISTMAS TREE» STAINING : These tests allow for the detection of sperm.
2) PSA TEST: PSA test is used to detect the presence of very small amounts of seminal fluid even in the absence of spermatozoa (in cases of vasectomized or azoospermic males) by measuring the levels of the prostate-specific antigen protein that is produced by the cells of the prostate gland.
3)«CHRISTMAS TREE» STAINING:Detection of sperm under microscope can be performed by applying the «Christmas Tree» stain.