ΕUROPEAN CENTER FOR GENETICS & DNA IDENTIFICATION

Y-STR ANALYSIS
DNAlogy
Instructions
INSTRUCTIONS FOR BUCCAL CELL COLLECTION: DNALOGY-KIT
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In the course of sampling, DNAlogy offers you free-of charge the DNAlogy-kit including the sterilized cotton swab, sampling instructions and a consent/order form for the applicant.
The aim of sampling is to collect as many cheek cells as possible and to transfer them to the sterilized cotton swab, which is going to protect DNA until its transfer to our laboratory.
Attention: Avoid any contact with the cotton tip end of the swabs.
Do not eat, drink or smoke for at least 30 minutes before sampling.
For the sampling procedure, each individual should use 2 swabs.

Step 1
Label the swabs with a unique identifying name and sampling date.

Step 2
Carefully open the sterile swab packaging holding the red plastic applicator end and remove the swab from the packaging.

Step 3
Sampling: Rub the cotton tip of the swab firmly against the inside of both cheeks several times (20-30 seconds) in each direction.

Step 4
Air dry the swabs for at least 1 hour at room temperature while keeping them protected from any source of contamination. When dry, place the swabs back in their packaging. Repeat the steps 1-4 with the second swab

Step 5
Send the swabs back to DNAlogy along with the completed questionnaire (consent and order form) by using the reply envelope.


Y-STR ANALYSIS


Y-STR analysis targets polymorphic regions of the Y-chromosome. The areas with Y-STR are specifically designated areas of the Y-chromosome. The Y chromosome is hereditary from the paternal gene, which means that all male offspring have the same y gene

Y-STR analysis is applied in:

Α) Sexual assault cases and rapes:
1. It allows the listing of people who had sex with the victim.
2. Identification of the violator even when the female DNA sample is more than the male DNA in a mixed sample.

Β) Identification of dead bodies/ Paternity testing when the father is deceased :
1. Y-STR analysis allows identification of dead bodies and also identification of a person whose close relatives (parents, brothers/sisters) are deceased or missing by analyzing paternally-related males .