ΕUROPEAN CENTER FOR GENETICS & DNA IDENTIFICATION

Y-STR ANALYSIS
DNAlogy
Instructions
INSTRUCTIONS FOR BUCCAL CELL COLLECTION: DNALOGY-KIT
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In the course of sampling, DNAlogy offers you free-of charge the DNAlogy-kit including the sterilized cotton swab, sampling instructions and a consent/order form for the applicant.
The aim of sampling is to collect as many cheek cells as possible and to transfer them to the sterilized cotton swab, which is going to protect DNA until its transfer to our laboratory.
Attention: Avoid any contact with the cotton tip end of the swabs.
Do not eat, drink or smoke for at least 30 minutes before sampling.
For the sampling procedure, each individual should use 2 swabs.

Step 1
Label the collection tube of swab with the code "Sample 1" and the sampling date.

Step 2
Carefully open the first collection tube by holding the red plastic handle end and carefully remove the sterile swab for buccal cell collection.

Step 3
Sampling: Holding the red plastic handle end of swab, place the cotton tip of the swab inside the cheek and rub it firmly in every direction several times (20 seconds in each cheek). Repeat steps 1-3 with the second collection tube with the code "Sample 1".

Step 4
Air dry the swabs for at least 3 hours (no less) at room temperature while keeping them protected from any source of contamination. When dry, place the swabs back in the collection tubes.

Step 5
Place the two collection tubes containing the biological material to be analyzed in the reply envelope and send it back to DNAlogy along with the completed Consent Form.


Y-STR ANALYSIS


Y-STR analysis targets polymorphic regions of the Y-chromosome. The areas with Y-STR are specifically designated areas of the Y-chromosome. The Y chromosome is hereditary from the paternal gene, which means that all male offspring have the same y gene

Y-STR analysis is applied in:

Α) Sexual assault cases and rapes:
1. It allows the listing of people who had sex with the victim.
2. Identification of the violator even when the female DNA sample is more than the male DNA in a mixed sample.

Β) Identification of dead bodies/ Paternity testing when the father is deceased :
1. Y-STR analysis allows identification of dead bodies and also identification of a person whose close relatives (parents, brothers/sisters) are deceased or missing by analyzing paternally-related males .