ΕUROPEAN CENTER FOR GENETICS & DNA IDENTIFICATION

NUCLEAR DNA ANALYSIS
DNAlogy
Instructions
INSTRUCTIONS FOR BUCCAL CELL COLLECTION: DNALOGY-KIT
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In the course of sampling, DNAlogy offers you free-of charge the DNAlogy-kit including the sterilized cotton swab, sampling instructions and a consent/order form for the applicant.
The aim of sampling is to collect as many cheek cells as possible and to transfer them to the sterilized cotton swab, which is going to protect DNA until its transfer to our laboratory.
Attention: Avoid any contact with the cotton tip end of the swabs.
Do not eat, drink or smoke for at least 30 minutes before sampling.
For the sampling procedure, each individual should use 2 swabs.

Step 1
Label the collection tube of swab with the code "Sample 1" and the sampling date.

Step 2
Carefully open the first collection tube by holding the red plastic handle end and carefully remove the sterile swab for buccal cell collection.

Step 3
Sampling: Holding the red plastic handle end of swab, place the cotton tip of the swab inside the cheek and rub it firmly in every direction several times (20 seconds in each cheek). Repeat steps 1-3 with the second collection tube with the code "Sample 1".

Step 4
Air dry the swabs for at least 3 hours (no less) at room temperature while keeping them protected from any source of contamination. When dry, place the swabs back in the collection tubes.

Step 5
Place the two collection tubes containing the biological material to be analyzed in the reply envelope and send it back to DNAlogy along with the completed Consent Form.

NUCLEAR DNA ANALYSIS

Nuclear DNA is found within all cells (apart from red blood cells) and is organized into long structures of chromosomes (see § what DNA is). DNA is inherited from both parents and allows for the genetic identification of an individual.
The technology used for genetic profiling studies specific regions (loci) of the DNA, called STRs (Short Tandem Repeats: usually tetrameric repeating sequences), that are popular due to their high level of variability that allows one to distinguish one DNA profile form another. The number of repeats of the STR sequence is determined by each chromosome (half of which is inherited from the father and half from the mother).The analysis of several regions of DNA (at least16) determines the type of STR genetic fingerprint (gene profile) of each individual, which statistically occurs only once in 180 billion people and is now the most accepted method worldwide.

-Thanks to the advanced technology, DNAlogy can extract nuclear DNA from any biological material (blood, saliva, semen, skin, sweat, hair, nasal secretions, etc.) and from other possible sources (cigarette butts, toothbrushes, chewing gum, food, bottles , glasses, hair combs, clothing, hoods, gloves, guns, knives, underwear, condoms, etc.). 

Analysis of nuclear DNA is used:

  • Worldwide, for the creation of national archives of genetic fingerprints (genetic identity)
  • test biological relationships ( paternity test, maternity test, brothers, the identification of bodies ...)
  • In sexual assault cases (identification of nuclear DNA from lingerie, condoms, sheets ...) 
  • In various crimes (identification of nuclear DNA from chewing gum, cigarette butts, fruit, food, bottles, glasses, hair combs, gloves) 
  • In cases of armed robberies (nuclear DNA identification from hoods, weapons, bullets)

- Nuclear DNA is the most determinative type of DNA i.e. it is more informative in DNA identification. The more concentrated the biological samples are (blood, sperm, saliva, sweat), the more chances we have to extract nuclear DNA. However nuclear DNA is more sensitive to environmental influences and therefore samples should be protected from humidity, light and contaminants.

 Contact us to help you collect, maintain and dispatch your samples to our laboratory.