ΕUROPEAN CENTER FOR GENETICS & DNA IDENTIFICATION

Instructions
INSTRUCTIONS FOR BUCCAL CELL COLLECTION: DNALOGY-KIT
39352129

In the course of sampling, DNAlogy offers you free-of charge the DNAlogy-kit including the sterilized cotton swab, sampling instructions and a consent/order form for the applicant.
The aim of sampling is to collect as many cheek cells as possible and to transfer them to the sterilized cotton swab, which is going to protect DNA until its transfer to our laboratory.
Attention: Avoid any contact with the cotton tip end of the swabs.
Do not eat, drink or smoke for at least 30 minutes before sampling.
For the sampling procedure, each individual should use 2 swabs.

Step 1
Label the collection tube of swab with the code "Sample 1" and the sampling date.

Step 2
Carefully open the first collection tube by holding the red plastic handle end and carefully remove the sterile swab for buccal cell collection.

Step 3
Sampling: Holding the red plastic handle end of swab, place the cotton tip of the swab inside the cheek and rub it firmly in every direction several times (20 seconds in each cheek). Repeat steps 1-3 with the second collection tube with the code "Sample 1".

Step 4
Air dry the swabs for at least 3 hours (no less) at room temperature while keeping them protected from any source of contamination. When dry, place the swabs back in the collection tubes.

Step 5
Place the two collection tubes containing the biological material to be analyzed in the reply envelope and send it back to DNAlogy along with the completed Consent Form.

GENERAL INFORMATION

WHAT DNA IS

BASIC BIOCHEMICAL AND GENETIC KNOWLEDGE

 

WHAT IS A GENETIC FINGERPRINT?

A Genetic fingerprint is a person’s genetic identity which is composed of DNA sequences that are distinguishable from one individual to another.

WHAT DNA IS?

DNA (deoxyribonucleic acid) is the genetic material of all living organisms and is organized into stretches of genes that contain the instruction for making all the thousand of proteins that are found in a cell.

Out of the thousand of important properties of living organisms, the most important is that of perpetuation. For this reason, the body must have a full description of himself ,which is equivalent to a manual, to explain the construction of duplication.. Within the cell, DNA is organized into long structures called chromosomes, which contain all the necessary information for DNA’s replication.

DNA consists of four building blocks called nucleotides, which are composed of a nitrogenous base (Adenine (A), Thymine (T), Guanine (G) and Cytosine (C)) and one to three phosphate groups.

DNA exists as a double helix with a pair of molecules that are held tightly together (two strands) and twisted in opposite directions. The two strands are held together by hydrogen bonds between the bases of the strands.

The sequence of the DNA base pairs is formed as a code that spell the genetic words, the genes, which tell our cells what to do. This is like a manual written with 4 letters (A, T, G, C) of which only these 4 letters appear in a certain order (sequence) to form words or sentences.

 

WHERE IS DNA FOUND?

DNA is found in most cells in the human body (approx. 100 trillion), blood, tissues, organs: teeth, hair, saliva, mucus, bones etc

 WHAT ARE THE TYPES OF DNA ?

There are 2 kinds of DNA: nuclear DNA and mitochondrial DNA

30/01/2010 17:09